Conventional Test Method:

No specific conventional test method is superseded

Alternative Test Method:

Name & Description:

Saccharomyces cerevisiae Gene Mutation Assay

This assay may be used to measure gene mutation in yeast. A variety of haploid and diploid strains of the yeast can be used to measure the production of gene mutations induced by chemical agents. Stationary or growing cells are exposed to the test compound, with and without an exogenous mammalian metabolic activation system. After incubation, plates are scored for survival and the induction of gene mutation.

Validation Status:

No information

Regulatory Status:

OECD: TG 480 (1986)

Effect or Potential Effect on Animal Use:

Reduction
(part of a tiered testing strategy that results in use of  fewer animals)

References:

• OECD (1986) OECD Guideline for Testing of Chemicals. Test Guideline 480: Genetic Toxicology Saccharomyces cerevisiae, Gene Mutation Assay.

Information Last Reviewed:

May 2012