| No specific conventional test method is superseded |
Bacterial Reverse Mutation Test (Ames test)
Description and references |
No information |
OECD: TG 471 (1997) US: Approved for the safety testing of food by US FDA (2000) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
Saccharomyces cerevisiae Gene Mutation Assay
Description and references |
No information |
OECD: TG 480 (1986) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
Saccharomyces cerevisiae Mitotic Recombination Assay
Description and references |
No information |
OECD: TG 481 (1986) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
In Vitro Mammalian Chromosome Aberration Test
Description and references |
No information |
OECD: TG 473 (1997) US: EPA Health Effects Test Guideline POOTS 870.5375 (1998); and approved for the safety testing of food by US FDA (2003) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
In Vitro Mammalian Cell Gene Mutation Test
Description and references |
No information |
OECD: TG 476 (1997) US: EPA Health Effects Test Guideline OPPTS 870.5300 (1996) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
In Vitro Sister Chromatid Exchange (SCE) Test
(Non-lethal Mouse Model for Botulinum Toxin Potency Testing)
Description and references |
No information |
OECD: TG 479 (1986) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
In Vitro Unscheduled DNA Synthesis (UDS) in Mammalian Cells Test
Description and references |
No information |
OECD: TG 482 (1986) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
| In Vitro Mammalian Chromosome Aberration Test (OECD TG 473) |
In Vitro Micronucleus Test (MNT)
Description and references |
EU: Endorsed as valid by ESAC (2006) |
OECD: OECD TG 487 (2010)
EU: Approved for use as part of REACH (Registration, Evaluation, Authorisation and Restriction of Chemicals) (2006)
Under consideration for revised ICH guidelines |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
| No specific conventional test method is superseded |
In Vitro Comet Assay (Single-Cell Gel Electrophoresis [SCGE] Technique)
Description and references |
Japan: JaCVAM presently conducting validation |
OECD: new Test Guideline proposed |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
