Acute Eye Irritation/Corrosion
(Draize rabbit eye irritation test)
(OECD TG 405) |
Bovine Corneal Opacity and Permeability (BCOP) Test Method
Description and references |
OECD: validated
EU: Endorsed as valid by ESAC (2007)
US: Endorsed as valid by ICCVAM (2007) |
OECD: TG 437 (2013)
EU: Accepted for use to positively identify and label substances as severe eye irritants (R41) (2002)
US: Accepted as a screening test to detect ocular corrosives and severe irritants in a tiered testing strategy (2008) |
Reduction
(Substances identified as irritants by test will not progress to animal testing) |
June 2014 |
Isolated Chicken Eye (ICE) Test Method
Description and references |
EU: validated
US: Endorsed as valid by ICCVAM (2007) |
OECD: TG 438 (2013)
EU: Accepted for use to positively identify and label substances as severe eye irritants (R41) (2002)
US: Accepted as a screening test to detect ocular corrosives and severe irritants in a tiered testing strategy (2008) |
Reduction
(Substances identified as irritants by test will not progress to animal testing) |
June 2014 |
Hen’s Egg Test - Chorioallantoic Membrane (HET-CAM) Test Method
Description and references |
EU: Concluded not sufficiently validated by ESAC (2007)
US: Concluded not sufficiently validated by ICCVAM (2007) |
EU: Although not formally validated, accepted for use to positively identify and label substances as severe eye irritants (R41) (2002) |
Reduction
(Substances identified as irritants by test will not progress to animal testing) |
May 2012 |
Isolated Rabbit Eye (IRE) Test Method
Description and references |
EU: Concluded not sufficiently validated by ESAC (2007)
US: Concluded not sufficiently validated by ICCVAM (2007) |
EU: Although not formally validated, accepted for use to positively identify and label substances as severe eye irritants (R41)(2002) |
Reduction
(Substances identified as irritants by test will not progress to animal testing) |
May 2012 |
Slug Mucosal Irritation (SMI) Assay
Description and references |
EU: Prevalidation study results have been submitted to ECVAM for evaluation. |
No information |
Replacement |
May 2012 |
Fluorescein Leakage Test
Description and references |
EU: Endorsed as valid by ESAC (2009) |
OECD: OECD TG 460 (2012) |
Redection
(Substances identified as irritants by test will not progress to animal testing) |
October 2012 |
Cytosensor Microphysiometer (CM) modified
Description and references |
EU: Endorsed as valid by ESAC (2009)
US: can be used as a screening test to identify some types of substances that may cause permanent or severe eye injuries (2010) |
OECD: Draft new TG (2011) |
Redection
(Substances identified as irritants by test will not progress to animal testing) |
May 2012 |
