Acute Oral LD50
(formerly OECD TG 401, now deleted as a test method) |
Fixed-Dose Procedure
Description and references |
EU: Endorsed as equivalent to scientifically validated by ESAC
US: None
|
OECD: TG 420 (2001) |
Reduction
(Reduces the number of animals used per test from 25 to 5-9)
Refinement
(Does not rely on death as an endpoint) |
May 2012 |
Acute Toxic Class Method
Description and references |
EU: Endorsed as equivalent to scientifically validated by ESAC
US: none
|
OECD: TG 423 (2001) |
Reduction
(Reduces the number of animals used per test from 25 to 5-9) |
May 2012 |
Up-and-Down Procedure
Description and references |
EU: Endorsed as equivalent to scientifically validated by ESAC (2006)
US: Endorsed as valid by ICCVAM
|
OECD: TG 425 (2008) |
Reduction
(Reduces the number of animals used per test from 25 to 5-9) |
May 2012 |
Neutral Red Uptake (NRU) test with human cells (normal human epidermal keratinocytes (NHK))
(NHK NRU assay)
Description and references |
US: Endorsed as valid by ICCVAM for the purpose of determining starting doses for in vivo acute oral toxicity tests (2006) |
US: Recommended by ICCVAM to regulatory agencies for approval (2008)
OECD: GD 129 (2010) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
Neutral Red Uptake (NRU) test with rodent cells (mouse 3T3 fibroblasts)
(3T3 NRU assay)
Description and references |
US: Endorsed as valid by ICCVAM for the purpose of determining starting doses for in vivo acute oral toxicity tests (2006) |
US: Recommended by ICCVAM to regulatory agencies for approval (2008)
OECD: GD 129 (2010) |
Reduction
(part of a tiered testing strategy that results in use of fewer animals) |
May 2012 |
