Skin Sensitization
(Guinea Pig Maximization Test;
Buehler Test)
(OECD TG 406) |
Classical Local Lymph Node Assay (LLNA)
Description and references |
EU: Endorsed as valid by ECVAM (2000)
US: Endorsed as valid by ICCVAM and recommended for use as a stand-alone substitute for the guinea pig sensitization test (1999)
|
OECD: TG 429 (2010)
International Standards Organization [ISO] 10993-10: Tests for Irritation and Sensitization (2002)
EU: Method B.42 in Annex to 440/2008/EC (2004, 2008)
US: EPA Health Effect Testing Guidelines on Skin Sensitization (2003)
|
Reduction
Refinement
(Does not require dermal hypersensitivity reactions be elicited in the animals; does not require administration of an adjuvant which causes pain and distress)
|
May 2012 |
Classical Local Lymph Node Assay (LLNA)
(OECD TG 429) |
rLLNA (Reduced Local Lymph Node Assay: Limit Dose Procedure)
Description and references |
EU: Endorsed by ECVAM as a substitute method for the LLNA to distinguish between skin sensitizers and non-sensitizers when used as part of a tiered testing strategy (2007).
US: When dose-response information is not required, ICCVAM recommends use of the LLNA limit dose procedure as for hazard identification to identify sensitizers and non-sensitizers.
When dose-response information is required, ICCVAM recommended rLLNA be used as the initial testing procedure to identify sensitizers and non-sensitizers.
|
OECD: TG 429 (2010) |
Reduction
(Chemicals that test negative in the rLLNA will not proceed to the full LLNA test and therefore reduce animal use) |
May 2012 |
